Intended learning outcomes (knowledge, skills and competences to be developed by the students):
The aim of the Laboratory Rotations is to prepare students for scientific research work in the laboratory, through the knowledge of basic and transversal techniques in several areas, from molecular and cellular biology, biochemistry and genetics, to microbiology and immunology, to computational biomedicine, among others.
Students will also be challenged to acquire knowledge about the relevance of biobanks as essential infrastructures to support basih and clinical research.
In addition to training in the interpretation and experimental execution of the protocols, the skills to be acquired in this CU include the organization of scientific data and the preparation of scientific articles Students will be offered an in-depth study of the contents developed for the pursuit of personal goals, with suggestions for additional readings.
Syllabus:
The following topics will be addressed in this CU:
Computational Biomedicine
- Computational techniques for the proposal of Biomarker panels.
- Computational techniques to elucidate molecular mechanisms in health and disease.
- In silico mutation analysis: assessment of the structural and functional impact of mutations, prediction of pathogenicity.
Cell Culture
- Basic cell culture techniques.
- Cytotoxicity and Biocompatibility Assays.
Applied Proteomics, Genomics and Metabolomics (Epi)genetics
- Identification of polymorphisms and genotyping.
- Determination of gene expression and methylation.
Biobanks and Biological Samples Management
- Operationalization and management of biobanks.
- Importance of biobanks in clinical research and precision medicine.
Microbiology
- Collection and processing of samples for Microbiological analysis.
- Techniques for identification and quantification of microorganisms.
- Antimicrobial activity tests.